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spheroid formation medium  (STEMCELL Technologies Inc)

 
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    STEMCELL Technologies Inc spheroid formation medium
    Spheroid Formation Medium, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/spheroid formation medium/product/STEMCELL Technologies Inc
    Average 90 stars, based on 1 article reviews
    spheroid formation medium - by Bioz Stars, 2026-03
    90/100 stars

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    The effect of N-terminal-truncated PIP5K1α on the proliferation and migratory ability of PIP5K1αΔN C4-2 cells. (A) Immunoblot analysis on the expression of trunctated PIP5K1α in PIP5K1αΔN C4-2 cells (PIP5K1αΔN) or full-length PIP5K1α in SG C4-2 cells (SG) is shown. The antibody against full-length PIP5K1α was used. Quantification of the expression level of PIP5K1αΔN and PIP5K1α in the cells is shown. (B) Representative image of the quantitative RT-PCR to show mRNA expression of WT PIP5K1A and truncated PIP5K1A in SG cells and in PIP5K1αΔN cells, respectively. (C) Immunoblot analysis on the expression of PIP5K1α and PIP5K1αΔN in SG cells and PIP5K1αΔN cells that were treated with MG132 or vehicle control. Quantifications of the immunoblots for expression of PIP5K1αΔN and PIP5K1α are shown in the right panel. (D) Non-radioactive MTS reagent was used to measure the proliferation rate of SG cells and PIP5K1αΔN cells. (E) SG cells and PIP5K1αΔN cells were subjected to the trans-well Boyden chamber migration assays. The relative counts of the migrated cells are shown. (F) SG cells and PIP5K1αΔN cells were subjected to the tumorspheroid <t>formation</t> assay to assess the single-cell derived <t>tumor-spheroid</t> formation and growth. Representative images of tumor-spheroids derived from SG cells and PIP5K1αΔN cells are shown. The relative counts of tumor spheroids from each group are shown. Data in this figure is presented as the average of three independent experiments (±SE). Student’s t -test was used in the analysis. ** p < 0.01 is indicated.
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    The effect of N-terminal-truncated PIP5K1α on the proliferation and migratory ability of PIP5K1αΔN C4-2 cells. (A) Immunoblot analysis on the expression of trunctated PIP5K1α in PIP5K1αΔN C4-2 cells (PIP5K1αΔN) or full-length PIP5K1α in SG C4-2 cells (SG) is shown. The antibody against full-length PIP5K1α was used. Quantification of the expression level of PIP5K1αΔN and PIP5K1α in the cells is shown. (B) Representative image of the quantitative RT-PCR to show mRNA expression of WT PIP5K1A and truncated PIP5K1A in SG cells and in PIP5K1αΔN cells, respectively. (C) Immunoblot analysis on the expression of PIP5K1α and PIP5K1αΔN in SG cells and PIP5K1αΔN cells that were treated with MG132 or vehicle control. Quantifications of the immunoblots for expression of PIP5K1αΔN and PIP5K1α are shown in the right panel. (D) Non-radioactive MTS reagent was used to measure the proliferation rate of SG cells and PIP5K1αΔN cells. (E) SG cells and PIP5K1αΔN cells were subjected to the trans-well Boyden chamber migration assays. The relative counts of the migrated cells are shown. (F) SG cells and PIP5K1αΔN cells were subjected to the tumorspheroid <t>formation</t> assay to assess the single-cell derived <t>tumor-spheroid</t> formation and growth. Representative images of tumor-spheroids derived from SG cells and PIP5K1αΔN cells are shown. The relative counts of tumor spheroids from each group are shown. Data in this figure is presented as the average of three independent experiments (±SE). Student’s t -test was used in the analysis. ** p < 0.01 is indicated.
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    The effect of N-terminal-truncated PIP5K1α on the proliferation and migratory ability of PIP5K1αΔN C4-2 cells. (A) Immunoblot analysis on the expression of trunctated PIP5K1α in PIP5K1αΔN C4-2 cells (PIP5K1αΔN) or full-length PIP5K1α in SG C4-2 cells (SG) is shown. The antibody against full-length PIP5K1α was used. Quantification of the expression level of PIP5K1αΔN and PIP5K1α in the cells is shown. (B) Representative image of the quantitative RT-PCR to show mRNA expression of WT PIP5K1A and truncated PIP5K1A in SG cells and in PIP5K1αΔN cells, respectively. (C) Immunoblot analysis on the expression of PIP5K1α and PIP5K1αΔN in SG cells and PIP5K1αΔN cells that were treated with MG132 or vehicle control. Quantifications of the immunoblots for expression of PIP5K1αΔN and PIP5K1α are shown in the right panel. (D) Non-radioactive MTS reagent was used to measure the proliferation rate of SG cells and PIP5K1αΔN cells. (E) SG cells and PIP5K1αΔN cells were subjected to the trans-well Boyden chamber migration assays. The relative counts of the migrated cells are shown. (F) SG cells and PIP5K1αΔN cells were subjected to the tumorspheroid <t>formation</t> assay to assess the single-cell derived <t>tumor-spheroid</t> formation and growth. Representative images of tumor-spheroids derived from SG cells and PIP5K1αΔN cells are shown. The relative counts of tumor spheroids from each group are shown. Data in this figure is presented as the average of three independent experiments (±SE). Student’s t -test was used in the analysis. ** p < 0.01 is indicated.
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    The effect of N-terminal-truncated PIP5K1α on the proliferation and migratory ability of PIP5K1αΔN C4-2 cells. (A) Immunoblot analysis on the expression of trunctated PIP5K1α in PIP5K1αΔN C4-2 cells (PIP5K1αΔN) or full-length PIP5K1α in SG C4-2 cells (SG) is shown. The antibody against full-length PIP5K1α was used. Quantification of the expression level of PIP5K1αΔN and PIP5K1α in the cells is shown. (B) Representative image of the quantitative RT-PCR to show mRNA expression of WT PIP5K1A and truncated PIP5K1A in SG cells and in PIP5K1αΔN cells, respectively. (C) Immunoblot analysis on the expression of PIP5K1α and PIP5K1αΔN in SG cells and PIP5K1αΔN cells that were treated with MG132 or vehicle control. Quantifications of the immunoblots for expression of PIP5K1αΔN and PIP5K1α are shown in the right panel. (D) Non-radioactive MTS reagent was used to measure the proliferation rate of SG cells and PIP5K1αΔN cells. (E) SG cells and PIP5K1αΔN cells were subjected to the trans-well Boyden chamber migration assays. The relative counts of the migrated cells are shown. (F) SG cells and PIP5K1αΔN cells were subjected to the tumorspheroid <t>formation</t> assay to assess the single-cell derived <t>tumor-spheroid</t> formation and growth. Representative images of tumor-spheroids derived from SG cells and PIP5K1αΔN cells are shown. The relative counts of tumor spheroids from each group are shown. Data in this figure is presented as the average of three independent experiments (±SE). Student’s t -test was used in the analysis. ** p < 0.01 is indicated.
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    The effect of N-terminal-truncated PIP5K1α on the proliferation and migratory ability of PIP5K1αΔN C4-2 cells. (A) Immunoblot analysis on the expression of trunctated PIP5K1α in PIP5K1αΔN C4-2 cells (PIP5K1αΔN) or full-length PIP5K1α in SG C4-2 cells (SG) is shown. The antibody against full-length PIP5K1α was used. Quantification of the expression level of PIP5K1αΔN and PIP5K1α in the cells is shown. (B) Representative image of the quantitative RT-PCR to show mRNA expression of WT PIP5K1A and truncated PIP5K1A in SG cells and in PIP5K1αΔN cells, respectively. (C) Immunoblot analysis on the expression of PIP5K1α and PIP5K1αΔN in SG cells and PIP5K1αΔN cells that were treated with MG132 or vehicle control. Quantifications of the immunoblots for expression of PIP5K1αΔN and PIP5K1α are shown in the right panel. (D) Non-radioactive MTS reagent was used to measure the proliferation rate of SG cells and PIP5K1αΔN cells. (E) SG cells and PIP5K1αΔN cells were subjected to the trans-well Boyden chamber migration assays. The relative counts of the migrated cells are shown. (F) SG cells and PIP5K1αΔN cells were subjected to the tumorspheroid formation assay to assess the single-cell derived tumor-spheroid formation and growth. Representative images of tumor-spheroids derived from SG cells and PIP5K1αΔN cells are shown. The relative counts of tumor spheroids from each group are shown. Data in this figure is presented as the average of three independent experiments (±SE). Student’s t -test was used in the analysis. ** p < 0.01 is indicated.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: PIP5K1α is Required for Promoting Tumor Progression in Castration-Resistant Prostate Cancer

    doi: 10.3389/fcell.2022.798590

    Figure Lengend Snippet: The effect of N-terminal-truncated PIP5K1α on the proliferation and migratory ability of PIP5K1αΔN C4-2 cells. (A) Immunoblot analysis on the expression of trunctated PIP5K1α in PIP5K1αΔN C4-2 cells (PIP5K1αΔN) or full-length PIP5K1α in SG C4-2 cells (SG) is shown. The antibody against full-length PIP5K1α was used. Quantification of the expression level of PIP5K1αΔN and PIP5K1α in the cells is shown. (B) Representative image of the quantitative RT-PCR to show mRNA expression of WT PIP5K1A and truncated PIP5K1A in SG cells and in PIP5K1αΔN cells, respectively. (C) Immunoblot analysis on the expression of PIP5K1α and PIP5K1αΔN in SG cells and PIP5K1αΔN cells that were treated with MG132 or vehicle control. Quantifications of the immunoblots for expression of PIP5K1αΔN and PIP5K1α are shown in the right panel. (D) Non-radioactive MTS reagent was used to measure the proliferation rate of SG cells and PIP5K1αΔN cells. (E) SG cells and PIP5K1αΔN cells were subjected to the trans-well Boyden chamber migration assays. The relative counts of the migrated cells are shown. (F) SG cells and PIP5K1αΔN cells were subjected to the tumorspheroid formation assay to assess the single-cell derived tumor-spheroid formation and growth. Representative images of tumor-spheroids derived from SG cells and PIP5K1αΔN cells are shown. The relative counts of tumor spheroids from each group are shown. Data in this figure is presented as the average of three independent experiments (±SE). Student’s t -test was used in the analysis. ** p < 0.01 is indicated.

    Article Snippet: PCa cells were made in single-cell suspensions and 1 × 10 5 cells were cultured in suspension in 2 ml of tumor spheroid formation medium (Cat#20141-500, promab Cancer Stem PremiumTM).

    Techniques: Western Blot, Expressing, Quantitative RT-PCR, Control, Migration, Tube Formation Assay, Derivative Assay